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Distribution of ceramide and IGF-1R on the cell membrane. (A) Distribution of ceramide was obtained by immunostaining. (B) Distribution of IGF-1R was obtained by live staining. IGF-1R, insulin-like growth factor-1 receptor. Scale bar, 50 µm.

Journal: Oncology Letters

Article Title: Novel sphingomyelin biomarkers for brain glioma and associated regulation research on the PI3K/Akt signaling pathway

doi: 10.3892/ol.2019.10946

Figure Lengend Snippet: Distribution of ceramide and IGF-1R on the cell membrane. (A) Distribution of ceramide was obtained by immunostaining. (B) Distribution of IGF-1R was obtained by live staining. IGF-1R, insulin-like growth factor-1 receptor. Scale bar, 50 µm.

Article Snippet: The fluorescent antibody anti-human insulin-like growth factor-1 receptor (IGF-1R; eBioscience; cat. no. 16-8849; 1:20) was added to the DMEM containing 2% FBS.

Techniques: Immunostaining, Staining

Movement of IGF-1R. The distribution status of IGF-1R on the cell membrane was recorded by live staining technology. The movement track of IGF-1R can be observed with the laser scanning confocal microscopy at 0, 5, 10 and 20 min. IGF-1R, insulin-like growth factor-1 receptor. Scale bar, 50 µm.

Journal: Oncology Letters

Article Title: Novel sphingomyelin biomarkers for brain glioma and associated regulation research on the PI3K/Akt signaling pathway

doi: 10.3892/ol.2019.10946

Figure Lengend Snippet: Movement of IGF-1R. The distribution status of IGF-1R on the cell membrane was recorded by live staining technology. The movement track of IGF-1R can be observed with the laser scanning confocal microscopy at 0, 5, 10 and 20 min. IGF-1R, insulin-like growth factor-1 receptor. Scale bar, 50 µm.

Article Snippet: The fluorescent antibody anti-human insulin-like growth factor-1 receptor (IGF-1R; eBioscience; cat. no. 16-8849; 1:20) was added to the DMEM containing 2% FBS.

Techniques: Staining, Confocal Microscopy

Expression of IGF-1R on the cell surface. The immunostaining results of two groups of glioma U373-MG cells are shown. Cells in the two groups were treated with insulin. The influence of drug Desi on IGF-1R expression was observed by comparing the two groups. Desi, desipramine; IGF-1R, insulin-like growth factor-1 receptor. Scale bar, 50 µm.

Journal: Oncology Letters

Article Title: Novel sphingomyelin biomarkers for brain glioma and associated regulation research on the PI3K/Akt signaling pathway

doi: 10.3892/ol.2019.10946

Figure Lengend Snippet: Expression of IGF-1R on the cell surface. The immunostaining results of two groups of glioma U373-MG cells are shown. Cells in the two groups were treated with insulin. The influence of drug Desi on IGF-1R expression was observed by comparing the two groups. Desi, desipramine; IGF-1R, insulin-like growth factor-1 receptor. Scale bar, 50 µm.

Article Snippet: The fluorescent antibody anti-human insulin-like growth factor-1 receptor (IGF-1R; eBioscience; cat. no. 16-8849; 1:20) was added to the DMEM containing 2% FBS.

Techniques: Expressing, Immunostaining